00397865
Univerzita Komenského v Bratislave
Šafárikovo nám. 6, Bratislava, 81499, SVK
45341931
LifeTechnologies s.r.o.
Laurinská 18, Bratislava, 81101, SVK
Kity a materiál
izolácia, magnet, PCR, separácia, DNA, RNA
Tovar, Služba
1. The MethylMiner™ Methylated DNA Enrichment Kit – 1 ks
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Funkcia |
is for highly sensitive enrichment of methylated DNA for downstream analysis including PCR/qPCR based assays, bisuflite conversion followed by amplification, cloning, and sequencing, direct sequencing, library preparation for high-throughput sequencing, and sample-prep for DNA microarray analysis. Patterns of DNA methylation seem to be important for making determinations about development and diseases such as cancer. |
This kit enables superior enrichment and differential fractionation of double-stranded DNA based on CpG methylation density, with increased sensitivity over antibody-based methods. Fractionation permits important comparisons between samples and enables researchers to focus analysis on only the methylation densities of interest. • Partitioning with high affinity binding—at least 4-fold greater sensitivity than antibody-based methods; |
• Fractionation based on CpG methylation density—ds DNA capture is achieved with MBD2 protein and facilitates ligation of double-stranded adaptors for next-generation sequencing; • Rapid and easy elution with salt eliminates the need for proteinase K treatment and phenol:chloroform extraction; • Precise answers—fractionated DNA permits distinctions to be made regarding methylation status and density; • Fast protocol—completed in less than 4 hours; • Easy—simple handling with Dynabeads® the gold standard magnetic beads |
2. The DynaMag™-2 magnet – 1 ks
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Funkcia |
is optimized for efficient magnetic separation of all types of Dynabeads® (range 1–4.5 µm in diameter) in small (<2 mL) sample volumes. The DynaMag™-2 magnet combines a strong magnetic attraction for efficient separations in standard microcentrifuge tubes with a flexible and smart ergonomic design. • Optimal working volume: 10–2,000 µL; • Holds up to 16 standard 1.5–2 mL microcentrifuge tubes in numbered spaces; • Good control and visibility of your sample; • Top rack can be quickly removed from the magnet in the base, ready for resuspension, vortexing, rotation, or manual sample shaking |
3. The Qubit RNA HS Assay Kit – 3 ks
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Funkcia |
when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of low-abundance RNA samples. The assay is highly selective for RNA and will not quantitate DNA, protein, or free nucleotides. Common contaminants, such as salts, free nucleotides, solvents, detergents, or protein, are well-tolerated in the assay. The assay kit is designed to be accurate for RNA sample concentrations between 250 pg/µL and 100 ng/µL. |
The kit provides concentrated assay reagent, dilution buffer, and pre-diluted RNA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration using the Qubit Fluorometer. |
4. The Qubit 1X dsDNA HS (High-Sensitivity) Assay Kit – 4 ks
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Funkcia |
is designed specifically for use with the Qubit Fluorometer. The assay is highly selective for double-stranded DNA (dsDNA) over RNA and is designed to be accurate for initial sample concentrations from 10 pg/µL to 100 ng/µL. The "1X" dsDNA assay kit provides reagent and buffer in a formulation that is stable as a ready-to-use solution for up to one year after preparation. Simply add your sample (any volume between 1 µL and 20 µL is acceptable) and read the concentration using a Qubit 2.0, 3, or 4 fluorometer. |
Common contaminants such as salts, free nucleotides, solvents, detergents, and protein are well-tolerated in the assay |
Názov |
Vrátane dopravy na miesto plnenia |
Požaduje sa predložiť rozpis sadzby DPH a ceny s DPH alebo bez DPH, ktorá ako údaj v zmluve chýba v prípade plnenia zahŕňajúce rôzne sadzby DPH do 7 dní od uzavretia zmluvy |
Dodávateľ sa zaväzuje dodať tovar, ktorý v čase dodania nemá uplynutých viac ako 20% výrobcom stanovenej expiračnej doby a počas stanovenej expiračnej doby bude mať vlastnosti stanovené kvalitatívnymi a technickými parametrami. |
Predmet plnenia bude dodaný na základe objednávky v celom objeme a v termíne určenom objednávateľom. |
Konečná cena zahŕňa dovoz nepoškodeného tovaru na miesto plnenia. |
Fakturačné údaje budú poskytnuté víťaznému uchádzačovi v spojitosti s miestom dodania. Fakturácia v slovenskom jazyku (prílohou je dodací list). Splatnosť faktúry je 30 dní od jej predloženia a odsúhlasenia verejným obstarávateľom. |
Ústav molekulárnej biomedicíny, Sasinkova 4, 5.poschodie, 811 08 Bratislava, Bratislava, Bratislava, Bratislavský, Slovenská republika
05.03.2018 10:00 — 29.03.2018 14:00
1,00 Kompletný predmet zákazky
3 440,00
Názov | Merná jednotka | Výhodnejšia hodnota | Váha |
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Cena bez DPH | € | Menšia | 100 |
23.02.2018 14:06
28.02.2018 09:00
28.02.2018 09:15
20 min.
2 min.
Dátum predloženia | Hodnota | MJ | Kritérium |
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27.02.2018 13:33 - Vstupná ponuka | 3 063,97 | € | Cena bez DPH |
27.02.2018 13:33 - Najvýhodnejšia ponuka | 3 063,97 | € | Cena bez DPH |
Vážený používateľ,
v súvislosti s povinnosťou elektronizácie verejného obstarávania od 18.10.2018 a zároveň s cieľom vylepšenia používateľského komfortu sme pre vás pripravili novú verziu systému EKS, ktorá so sebou prináša: